肠道微生物研究可别忘了代谢组

Title：
Metabolomics of fecal samples: A practical consideration

DOI：
10.1016/j.tifs.2016.05.011

Abstract：
Background Metabolic profiling is becoming increasingly popular to identify subtle metabolic variations induced by diet alterations and to characterize the metabolic impact of variations of the gut microbiota. In this context, fecal samples, that contain unabsorbed metabolites, offer a direct access to the outcome of diet – gut microbiota metabolic interactions. Hence, they are a useful addition to measure the ensemble of endogenous and microbial metabolites, also referred to as the hyperbolome. Scope and approach Many reviews have focused on the metabolomics analysis of urine, plasma and tissue biopsies; yet the analysis of fecal samples presents some challenges that have received little attention. We propose here a short review of current practices and some practical considerations when analyzing fecal material using metabolic profiling of small polar molecules and lipidomics. Key findings and conclusions To allow for a complete coverage of the fecal metabolome, it is recommended to use a combination of analytical techniques that will measure both hydrophilic and hydrophobic metabolites. A clear set of guidelines to collect, prepare and analyze fecal material is urgently needed.

All Authors：
Silke Matysik,Caroline Ivanne Le Roy,Gerhard Liebisch,Sandrine Paule Claus

First Authors：
Sandrine Paule Claus

Correspondence：
Sandrine Paule Claus

内容要点：

1、包含未被吸收的代谢产物的粪便样品，为研究饮食-肠道菌群代谢之间的联系提供了便利；

2、粪便样品是很好的研究体内所有内源性及菌群代谢产物的材料；

3、许多综述关注了尿液、血浆及组织活检样品中的代谢组学；

4、本综述介绍了一些关于粪便样品中小极性分子及脂质的代谢组学的研究。