人体肠道菌群宏蛋白质组学分析方法
Title：
Metaproteomic analysis of human gut microbiota: where are we heading?
Abstract：
The human gut is home to complex microbial populations that change dynamically in response to various internal and external stimuli. The gut microbiota provides numerous functional benefits that are crucial for human health but in the setting of a disturbed equilibrium, the microbial community can cause deleterious outcomes such as diseases and cancers. Characterization of the functional activities of human gut microbiota is fundamental to understand their roles in human health and disease. Metaproteomics, which refers to the study of the entire protein collection of the microbial community in a given sample is an emerging area of research that provides informative details concerning functional aspects of the microbiota. In this mini review, we present a summary of the progress of metaproteomic analysis for studying the functional role of gut microbiota. This is followed by an overview of the experimental approaches focusing on fecal specimen for metaproteomics and is concluded by a discussion on the challenges and future directions of metaproteomic research.
All Authors：
Pey Yee Lee, Siok-Fong Chin, Hui-min Neoh, Rahman Jamal
First Authors：
Pey Yee Lee
Correspondence：
Siok-Fong Chin
内容要点：
人体肠道定居着复杂的微生物群，这些菌群因各种内外部刺激而动态变化；肠道菌群对人类健康起着重要的功能性作用，其平衡被打乱会引起疾病和癌症等有害结局，描述其特点是了解其对人类健康和疾病的基础；宏蛋白质组学，即收集所给样品的微生物的全部蛋白质进行研究，是提供关于微生物功能性方面的详细信息的新兴研究；本文总结了利用宏蛋白质组学研究肠道菌群功能性作用的进展，概括其粪便样本的研究方法，讨论了其研究前景和方向。