食用蜂胶改善葡聚糖硫酸钠诱导的结肠炎并调节西方饮食喂养大鼠的肠道菌群

Title：
Dietary Propolis Ameliorates Dextran Sulfate Sodium-Induced Colitis and Modulates the Gut Microbiota in Rats Fed a Western Diet

DOI：
10.3390/nu9080875

Abstract：
Propolis is an important hive product and considered beneficial to health. However, evidence of its potential for improving gut health is still lacking. Here we use rats to examine whether dietary supplementation with propolis could be used as a therapy for ulcerative colitis. Rats were fed with a Western style diet alone (controls) or supplemented with different amounts of Chinese propolis (0.1%, 0.2%, and 0.3%) to examine effects on acute colitis induced by 3% dextran sulphate sodium (DSS) in drinking water. Propolis at 0.3%, but not lower levels, significantly improved colitis symptoms compared with the control group, with a less pronounced disease activity index (DAI) (p < 0.001), a significant increase in colon length/weight ratio (p < 0.05) and an improved distal colon tissue structure as assessed by histology. Although short chain fatty acid levels in digesta were not altered by propolis supplementation, 16S rRNA phylogenetic sequencing revealed a significant increase in gut microbial diversity after 21 days of 0.3% propolis supplementation compared with controls including a significant increase in bacteria belonging to the Proteobacteria and Acidobacteria phyla. This is the first study to demonstrate that propolis can attenuate DSS-induced colitis and provides new insight into diet-microbiota interactions during inflammatory bowel disease.

All Authors：
Kai Wang,Xiaolu Jin,Mengmeng You,Wenli Tian,Richard K Le Leu,David L Topping,Michael A Conlon,Liming Wu,Fuliang Hu

First Authors：
Kai Wang,Xiaolu Jin

Correspondence：
Michael A Conlon,Liming Wu,Fuliang Hu

内容要点：

蜂胶是重要的蜂巢产品，对健康有益， 通过补充不同剂量的中国蜂胶（0.1％，0.2％和0.3％）喂养大鼠，评估对葡聚糖硫酸钠诱导的急性结肠炎的影响， 与对照相比，添加不低于0.3%的蜂胶显著改善结肠炎症状，显著降低疾病活动指数，显著增加结肠长度/重量比，并改善远端结肠组织结构。 补充蜂胶后，消化道中的短链脂肪酸水平没有改变，但添加0.3%蜂胶21天后，肠道菌群多样性显著增加，包括属于变形菌门和酸杆菌门的细菌显著增加。