利用28SrDNA测序技术可诊断罕见视网膜炎
Title：
The first case of Trypanosoma cruzi-associated retinitis in an immunocompromised host diagnosed by pan-organism PCR
Abstract：
We report the first case of Trypanosoma cruzi-associated retinitis which was diagnosed with the use of 28s ribosomal DNA sequencing. The case highlights the utility of broad-range molecular diagnostics for detecting rare and unsuspected ocular pathogens. Ocular involvement in Chagas' disease is also discussed.
All Authors：
Christopher D Conrady,Kimberly E Hanson,Sonia Mehra,Adrienne Carey,Marissa Larochelle,Akbar Shakoor
First Authors：
Christopher D Conrady
Correspondence：
Akbar Shakoor
摘要：
首次报道了利用28s rDNA测序技术，诊断出克氏锥虫相关视网膜炎的一个案例；患者是一位69岁的男性厄瓜多尔移民，曾患复发性多发性骨髓瘤，接受过多个化疗疗程及自体造血干细胞移植（HSCT）；患者被诊断为虹膜睫状体炎，使用靶向性PCR检测各种病原体均显示阴性；但利用泛真菌28S PCR检测到了与克氏锥虫序列具有97%相似性的序列；此案例提示，分子诊断学在检测罕见及未被怀疑的眼病原体中具有广泛用途。