结直肠癌中富集的7种细菌可作为诊断标记

Title：
Multi-cohort analysis of colorectal cancer metagenome identified altered bacteria across populations and universal bacterial markers

DOI：
10.1186/s40168-018-0451-2

Abstract：
Alterations of gut microbiota are associated with colorectal cancer (CRC) in different populations and several bacterial species were found to contribute to the tumorigenesis. The potential use of gut microbes as markers for early diagnosis has also been reported. However, cohort specific noises may distort the structure of microbial dysbiosis in CRC and lead to inconsistent results among studies. In this regard, our study targeted at exploring changes in gut microbiota that are universal across populations at species level.Based on the combined analysis of 526 metagenomic samples from Chinese, Austrian, American, and German and French cohorts, seven CRC-enriched bacteria (Bacteroides fragilis, Fusobacterium nucleatum, Porphyromonas asaccharolytica, Parvimonas micra, Prevotella intermedia, Alistipes finegoldii, and Thermanaerovibrio acidaminovorans) have been identified across populations. The seven enriched bacterial markers classified cases from controls with an area under the receiver-operating characteristics curve (AUC) of 0.80 across the different populations. Abundance correlation analysis demonstrated that CRC-enriched and CRC-depleted bacteria respectively formed their own mutualistic networks, in which the latter was disjointed in CRC. The CRC-enriched bacteria have been found to be correlated with lipopolysaccharide and energy biosynthetic pathways.Our study identified potential diagnostic bacterial markers that are robust across populations, indicating their potential universal use for non-invasive CRC diagnosis. We also elucidated the ecological networks and functional capacities of CRC-associated microbiota.

All Authors：
Zhenwei Dai, Olabisi Oluwabukola Coker, Geicho Nakatsu, William K K Wu, Liuyang Zhao, Zigui Chen, Francis K L Chan, Karsten Kristiansen, Joseph J Y Sung, Sunny Hei Wong, Jun Yu

First Authors：
Zhenwei Dai

Correspondence：
Sunny Hei Wong, Jun Yu

摘要：

      基于多个国家4个队列的526个宏基因组样本，鉴定出在结直肠癌（CRC）中富集的7个菌种： 脆弱拟杆菌、具核梭杆菌、不解糖卟啉单胞菌、微小小单胞菌、中间普雷沃菌、芬氏别样杆菌、食氨基酸热厌氧弧菌；用这7个菌种可在不同人群中区分CRC患者和健康对照，AUC为0.80（结合临床信息可提升至0.88）；丰度相关性分析表明，CRC中富集和减少的细菌分别形成各自的共生网络，二者互斥，且CRC中富集的细菌与脂多糖和能量生物合成途径相关。