马里乳杆菌APS1+节食治疗，能有效帮助小鼠减肥

Title：
A combination of Lactobacillus mali APS1 and dieting improved the efficacy of obesity treatment via manipulating gut microbiome in mice

DOI：
10.1038/s41598-018-23844-y

Abstract：
The difficulty of long-term management has produced a high rate of failure for obesity patients. Therefore, improving the efficacy of current obesity treatment is a significant goal. We hypothesized that combining a probiotic Lactobacillus mali APS1 intervention with dieting could improve the efficacy of obesity and hepatic steatosis treatment compared to dieting alone. Mice were fed a high-fat diet for 6 weeks and then treated with: saline + normal diet and APS1 + normal diet (NDAPS1) for 3 weeks. NDAPS1 accelerated body weight loss and reduced caloric intake and fat accumulation. The fecal microbiome showed that accelerating weight loss by NDAPS1 resulted in restoring intestinal microbiota toward a pre-obese state, with alteration of specific changes in the obesity-associated bacteria. APS1 manipulated the gut microbiome's obesity-associated metabolites, followed by regulation of lipid metabolism, enhancement of energy expenditure and inhibition of appetite. The specific hepatic metabolites induced by the APS1-manipulated gut microbiome also contributed to the amelioration of hepatic steatosis. Our results highlighted a possible microbiome and metabolome that contributed to accelerating weight loss following treatment with a combination of APS1 and dieting and suggested that probiotics could serve as a potential therapy for modulating physiological function and downstream of the microbiota.

All Authors：
Yung-Tsung Chen,Ning-Sun Yang,Yu-Chun Lin,Shang-Tse Ho,Kuan-Yi Li,Jin-Seng Lin,Je-Ruei Liu,Ming-Ju Chen

First Authors：
Yung-Tsung Chen

Correspondence：
Ming-Ju Chen

摘要：

       小鼠食用高脂食物6周，之后用正常饮食+生理盐水（对照）或马里乳杆菌APS1+正常饮食（NDAPS1）治疗3周， 发现NDAPS1干预加速小鼠减重，减少能量摄入和脂肪积累，改善脂肪肝，促进肠道菌群向肥胖前状态转化，肥胖相关细菌分类群丰度改变，链球菌科和厌氧棍状菌属减少、马里乳杆菌增加； 肥胖相关菌群代谢物改变，短链脂肪酸增加，血清中11种代谢物水平改变，调节脂代谢、增加能耗、抑制食欲； 经肠-肝轴影响肝脏相关代谢物，最终缓解脂肪肝。